The Austin Blind Salamander is known only from Barton Springs.  Unlike the Barton Springs Salamander, Austin Blind Salamanders are not typically seen near the surface. Austin Blind Salamanders occupy the habitat below the surface of the springs, where their unique adaptations likely give them a selective advantage in a world of total darkness and limited food. They have been found in all four of the springs collectively known as Barton Springs: Parthenia in Barton Springs Pool, Old Mill (Sunken Garden), Eliza and Upper Barton.  Unfortunately, because their habitat is not readily accessible by humans, and they are only occasionally observed in the springs, very little is known about the natural history of this species.

Old Mill Spring (Sunken Garden) in Zilker Park is the site where the Austin Blind Salamander (Eurycea waterlooensis) was first collected.

Interestingly, another large spring in Texas is also home to a pair of salamander species: San Marcos Springs in San Marcos (Hays Co.) has surface-dwelling San Marcos Salamanders (Eurycea nana) as well as the subterranean Texas Blind Salamanders (Eurycea rathbuni).